Analysis of common abnormal results of biochemistry

Reaction curve fluctuates and beats

一: almost all reaction curves beat

1. Poor grounding of the power supply: poor grounding of the main power source, poor grounding of the photoelectric cell;

2, the reaction plate into the water: vacuum pump pressure seal ring wear, poor contact with the vacuum pump power supply, leakage at the pipe joints, leaking pipes, pipe plug waste liquid, a one-way valve is broken, broken cuvette;

3, stirring rod is not stirred: the mixing motor is bad, the mixing motor wire is in poor contact, and the stirring rod is against the bottom of the colorimetric cup;

4, light path: light bulb mounting, color cup mounting, temperature control pot mounting, lens mounting;

5. Abnormal addition of reagents: The reagent needle is blocked, the reagent syringe falls off, the reagent needle is detached at the joint, and the quick connector comes off;

6, disposable cuvette is not clean;

7. Direct sunlight reaction photoelectric system;

8, external interference factors: such as the engine, electric drill and so on.

二: only the individual wavelength response curve beat

Poor grounding of the power supply: poor grounding of the main power supply and poor grounding of the photocell.

三: only individual project response curves beat

Abnormal reagent: the reagent becomes turbid, discolored or the reagent is misplaced;

After the reagent is stirred, more bubbles are formed to block the light path.

Abnormal reaction curve
1, almost all the reaction curves are in the correct shape, the reaction curve is stable, but basically no reaction

The sample was not added: The sample needle was clogged, the sample syringe was peeled off, the sample needle joint fell off, and the quick connector came off.

2, the individual project response curve shape is correct, the response curve is stable, but basically no response

The second reagent is not added: The second reagent is misplaced

3, the individual project response curve shape changes, but the reaction curve is stable

Abnormal addition of reagents: The reagent plate fixing pin was detached and the reagent injector was leaking.

4, the shape of the response curve of individual items changes, rises or falls sharply, the response curve is stable, or small fluctuations

Abnormal reagents: poor performance or failure of reagents, most likely to occur in ALP, GGT, AMY and other items.

5. The shape of individual test response curves changes, and they rise or fall sharply, but the response curve is stable.

1. Cross-contamination among reagents: It is most likely to occur on projects such as TG, TC, Glu and Bun;

2. Abnormal sample: The sample contains certain drugs or interference components, which are most likely to occur in TB, DB and other items.

The response curve is normal and the result is poor repeatability
一: Almost all projects have poor repeatability

1. Abnormal sample addition: semi-clogging of the sample needle, air bubbles in the sample injector, loose seal at the sample needle connector, and strict closure of the sample needle inner wall valve;

2. Abnormal addition of reagents: Semi-plugged reagent needle, air bubbles in the reagent injector, loose seal at the reagent needle connector, and strict shut-off of the reagent needle inner wall valve;

3. Abnormal stirring: the stirring motor is bad, the stirring motor line is in poor contact, and the stirring rod is against the bottom of the cuvette;

4. Insufficient cleaning of reagents/samples inside and outside wall, large cross-contamination: pressure drop of the liquid pump and clogging of the second-stage filter;

5. The temperature of the reaction disk is unstable: the temperature of the reaction disk fluctuates greatly.

二 : the individual project is poorly repeatable

1. The reagent is misplaced;

2, reagent abnormalities: poor reagent performance or failure, the most likely to occur in ALP, GGT, AMY and other projects;

3. Abnormal project parameter settings: small sample size, short reaction time, wrong reaction method setting;

4. Calibration abnormality: the calibration solution concentration is set incorrectly and the calibration solution is invalid.

The response curve is normal and the result is zero or very low
一: Almost all projects have zero or very low results

1. Abnormal sample addition: plugged sample needle, air bubble in the sample syringe, loose seal at the sample needle joint, and strict shut-off of the sample needle inner wall valve;

2. Stirring anomaly: Stirring motor is bad, the stirring motor wire is in poor contact, and the stirring rod is against the bottom of the cuvette.
二: individual projects result in zero or very low

1, reagent blank test abnormalities;

2, reagent misplaced: in particular, the second reagent misplaced;

3, reagent failure or discoloration: the most likely to occur in ALP, GGT, AMY and other projects;

4, less reagent.

三: other project test results are negative

1, reagent blank test abnormalities;

2, reagent failure or discoloration;

3. Abnormal sample addition: plugged sample needle, air bubble in the sample injector, loose seal at the sample needle joint, and strict shut-off of the sample needle inner wall valve;

4, reagent misplaced;

5, the second reagent misplaced or less reagent.

The response curve is normal and the result falls outside the quality control

一,almost all project quality control results fall outside the scope of quality control

1. Control fluid misplaced;

2. The control fluid fails.

二:, the results of individual project quality control fall outside the scope of quality control
1, reagent failure;

2, reagent blank test abnormalities;

3, reagent misplaced;

4. The calibrator fluid and the quality control fluid are not matched.

The response curve is normal and the result is generally high or low
一: all results are generally high or low
1, cup blank overflow, cup blank AD value is 65535;

2. Stirring rod stirs abnormally: the mixing motor is bad and the stirring motor wire is in poor contact;

3, add sample abnormalities: sample needle plug, solenoid valve close, liquid surface detection is abnormal.

二, the results of individual projects are generally high or low

1, reagent blank test abnormalities;

2. Abnormal reagent: Poor reagent performance, expired, mixed, discolored, etc.

3. Calibration anomalies: Improper input of calibration solution concentration, misplacement of calibration solution, and calibration solution failure.

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